Growth and Toxin Production by Clostridium botulinum in Model Acldified Systems
1985; Wiley; Volume: 50; Issue: 4 Linguagem: Inglês
10.1111/j.1365-2621.1985.tb12989.x
ISSN1750-3841
AutoresNora Tsang, Laurie Post, Myron Solberg,
Tópico(s)Botulinum Toxin and Related Neurological Disorders
ResumoABSTRACT TPGY medium was acidified to pH 4.20, 4.60, 5.00 and 5.40 with acetic or citric acid. The media were inoculated with spores from three strains of C. botulinum type A, B or E. Growth, pH, and toxin production under anaerobic conditions were monitored for 8 wk. Spores of C. botulinum types A and B were incapable of outgrowth and toxin production at pH 4.60 or below when incubated at 35°C. Spores of C. botulinum type E were capable of growth and toxin production at 26°C in citric acid acidified systems at pH 4.20. Growth and toxin production were not detected below pH 5.00 when acetic acid was used.
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