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Bioelectric recognition assay (BERA)

2001; Elsevier BV; Volume: 16; Issue: 4-5 Linguagem: Inglês

10.1016/s0956-5663(01)00127-0

1873-4235

Spyridon Kintzios, Eleni Pistola, P. Panagiotopoulos, Morgane Bomsel, N. Alexandropoulos, F. Bem, G Ekonomou, J Biselis, Richard B. Levin,

Advanced biosensing and bioanalysis techniques

A novel biosensory method has been developed for the determination of various chemical and biological molecules by assessing their electrophysiological interactions with a group of cells and cell components immobilized in a gel matrix that preserves their 'physiological' functions. The method was applied for the detection of: (i) hepatitis C virus in human blood samples; (ii) plant viruses; and (iii) a herbicide (glyphosate) in aqueous solutions. It was able to rapidly (assay time 3-5 min) and specifically detect the molecules in question at a concentration lower than 100 pg/ml, among other compounds f similar structure. The potential use of BERA biosensors for a rapid and cost-efficient molecule determination without prior knowledge of a specific receptor-molecule interaction is discussed.