Application of the particle beam interface to high-performance liquid chromatography-thermal energy analysis and electron impact mass spectrometry for detection of non-volatile N-nitrosamines
1994; Elsevier BV; Volume: 688; Issue: 1-2 Linguagem: Inglês
10.1016/0021-9673(94)00894-9
ISSN1873-3778
AutoresStanley M. Billedeau, Thomas M. Heinze, Jon G. Wilkes, Harold C. Thompson,
Tópico(s)Water Treatment and Disinfection
ResumoInterest in the analysis of non-volatile N-nitrosamines has recently been renewed due to the development of several new reversed-phase HPLC interfaces to thermal energy analysis (TEA) or chemiluminescence detection. A new application of a counter flow gas diffusion cell (CFGDC)-based particle beam LC interface (Universal Interface, Vestec) is described for the HPLC-TEA analysis of the non-volatile N-nitrosamines, N-Nitrosodiethanolamine (NDELA) and N-nitrosomethyl-p-amino-2-ethylhexylbenzoate (NMPABAO). The interface incorporates a thermospray vaporizer, desolvation chamber, and CFGDC to reduce the LC effluent to a dry aerosol and a single-stage momentum separator to form a particle beam of the non-volatile analyte. Using this system, the LC-TEA response to NDELA was linear in the range 6–200 ng total amount injected. Several experiments are reported indicating the effect of thermospray tip temperature, He carrier flow-rate, and mobile phase composition on TEA response. Minimum detection limits (5 ng NDELA injected on column) are comparable to other LC-TEA interfacing methods. Several advantages over existing methodology which include ease of use, ruggedness and MS compatibility are discussed. Additional LC-particle beam MS data are reported indicating that full scan electron impact MS identification of the N-nitrosamine contaminants in cosmetics is possible for confirming TEA detection data.
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