The biosynthesis of long-chain fatty acids by lettuce chloroplast preparations
1963; Elsevier BV; Volume: 70; Linguagem: Inglês
10.1016/0006-3002(63)90715-7
ISSN1878-2434
Autores Tópico(s)Photosynthetic Processes and Mechanisms
Resumo1. It is demonstrated that the major site of biosynthesis of saturated fatty acids from C10 to C18 and of oleic acid in leaves is the chloroplast. 2. The co-factors required in the presence of light are ATP, CoA, Mg2+, CO2 and inorganic phosphate. 3. In the dark greatly diminished synthesis occurs despite the presence of a full complement of cofactors. 4. As in photosynthetic phosphorylation the synthesis of fatty acids is inhibited by NH3 and 3(p-chlorophenyl) 1,1-dimethyl urea but not by dinitrophenol. 5. Under anaerobic conditions the biosynthesis of oleic acid drops markedly but that of the saturated acids is relatively unaffected. 6. Isolated chloroplasts are less effective in utilising the C8, C10, C12 and C14 saturated fatty acids for the biosynthesis of oleic acid than is the intact leaf. Both malonic and acetic acids are, however, readily utilised.
Referência(s)