Induction of drug specific antibody and the controlled release of drug by 6-O-carboxymethyl-chitin
1994; Elsevier BV; Volume: 28; Issue: 1-3 Linguagem: Inglês
10.1016/0168-3659(94)90170-8
ISSN1873-4995
AutoresTokura Seiichi, Yoshiaki Miura, Johmen Masayoshi, Nishi Norio, Nishimura Shin-Ichiro,
Tópico(s)Glycosylation and Glycoproteins Research
ResumoBiopolymeric properties of a water-soluble and biodegradable chitin derivative, 6-O-carboxymethyl-chitin (CM-chitin), have been investigated to demonstrate the immunological function serving to induce a hapten-specific antibody and the chemotherapeutic function as a drug carrier of controlled release. When CM-chitin was linked by methamphetamine (MA) through a nonbiodegradable spacer, 1-aminobutane (MABA-CM-chitin), MA-specific antibody was produced by the subcutaneous injection, in rabbits, of MABA-CM-chitin in combination with Freund's complete adjuvant, and when injected without intense immunoadjuvant, MABA-CM-chitin oligomer was secreted into blood for more than 120 h. Two-step hydrolysis of pendant-type of polymeric drug was also investigated in order to design a more sophisticated drug delivery system. Phenylalanine-containing peptide spacers were designed to be hydrolysed by enzymes other than lysozyme as a model drug. In the model system, the chromophore-bound phenylalanine peptide spacer was stabilized against enzymatic hydrolysis until CM-chitin was hydrolysed by lysozyme.
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