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Kunitz-Type Trypsin Inhibitor Prevents LPS-Induced Increase of Cytosolic Free Ca2+ in Human Neutrophils and HUVEC Cells

1995; Elsevier BV; Volume: 207; Issue: 1 Linguagem: Inglês

10.1006/bbrc.1995.1191

1090-2104

Naoki Kanayama, Abdul H. Halim, Kayoko Maehara, Yasuhiro Kajiwara, Michio Fujie, Toshihiko Terao,

Cell Adhesion Molecules Research

The protease inhibitor part of inter-α trypsin inhibitor is identical to urinary trypsin inhibitor (UTI). Preincubation of neutrophils and HUVEC cells with UTI inhibited increase of cytosolic free Ca2+ induced by LPS. Increase of cytosolic free Ca2+ induced by LPS in the presence of EGTA was also inhibited by UTI. In contrast, UTI did not inhibit increase of cytosolic free Ca2+ in cells stimulated by Ca2+ ionophore with or without EGTA. The effects of nine synthetic peptides of UTI on the concentration of cytosolic free Ca2+ in the neutrophils induced by LPS were examined. Preincubation with a peptide of UTI domain 2, NLPIVRGPCRAFIQL (83-97), was completely inhibited by the increase of cytosolic free Ca2+ in neutrophils. This region is identical to the trypsin inhibitor site of UTI. We propose that a function of UTI other than as a protease inhibitor is in regulation of intracellular Ca2+ and that this is due to its trypsin inhibitor region.