Determination of the effect of r and rb mutations on the structure of amylose and amylopectin in pea (Pisum sativum L.)
1996; Elsevier BV; Volume: 29; Issue: 1 Linguagem: Inglês
10.1016/0144-8617(95)00155-7
ISSN1879-1344
AutoresJames R. Lloyd, C. Hedley, V.J. Bull, Stephen G. Ring,
Tópico(s)Phytase and its Applications
ResumoThe structure of starch from pea lines near-isogenic except for mutation at the r and rb loci was compared with that of the wild-type. The isolated starch polysaccharides were fractionated by size exclusion chromatography, and the constituent chain profile of the branched amylopectin components examined. A mutation at the rb locus had little effect on starch structure and composition. A mutation at the r locus (a genetic lesion affecting the activity of an isoform of starch branching enzyme) resulted in an increase in the amylose content of the starch and a change in the structure of amylopectin.
Referência(s)