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An improved general approach for cloning and characterizing telomeres: the protozoan parasite Trypanosoma cruzi as model organism

2002; Elsevier BV; Volume: 294; Issue: 1-2 Linguagem: Inglês

10.1016/s0378-1119(02)00768-0

1879-0038

Miguel Ángel Chiurillo, Márcia Regina Machado dos Santos, José Franco da Silveira, José Luis Ramı́rez,

Trypanosoma species research and implications

We here describe a general strategy for cloning and characterizing telomeric and sub-telomeric regions of the human protozoan parasite Trypanosoma cruzi. The use of a bacterial artificial chromosome vector and a telomeric adaptor produced stable telomeric recombinant clones with inserts ranging from 5 to 25 kb. Analysis of these recombinants provided unique landmarks for chromosomal mapping and sequencing and enabled us to derive a more accurate picture of T. cruzi telomeric organization.