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An antiglobulin microcytotoxicity assay to analyze non-complement fixing monoclonal antibodies to human histocompatibility antigens

1982; Elsevier BV; Volume: 49; Issue: 2 Linguagem: Inglês

10.1016/0022-1759(82)90270-8

1872-7905

Michele Pellegrino, Carlo Russo, Ah‐Kau Ng, A. Pellegrino, Soldano Ferrone,

T-cell and B-cell Immunology

An antiglobulin microcytotoxicity assay has been used to analyze non-complement fixing monoclonal antibodies to human histocompatibility antigens. The assay utilizes methodology similar to that of the widely used microcytotoxicity assay for HLA typing, requires low numbers of target cells and is suitable to test large numbers of samples. The sensitivity of the assay is influenced by the anti-mouse Ig antiserum used, by the sequence of addition of the various reagents and by the incubation time. The assay is suitable to screen supernatants of clones derived from hybridization experiments and to characterize the serological specificity of anti-HLA monoclonal antibodies.