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Sensitive high-performance liquid chromatographic determination of nifedipine in dog plasma using an automated sample preparation system with laboratory robot

1996; Elsevier BV; Volume: 686; Issue: 2 Linguagem: Inglês

10.1016/s0378-4347(96)00216-2

1872-812X

Viola Horváth, Andrea Hrabéczy-Páll, Zsuzsanna Niegreisz, E. Kocsi, George Horvai, Lajos Gödörházy, Antal Tolokán, Imre Klebovich, Katalin Balogh-Nemes,

HIV/AIDS drug development and treatment

Nifedipine, a calcium-channel blocking drug was analysed in dog plasma after oral dosing with two different formulations. Sample preparation was automated with a laboratory robot. Quantitative determination of the drug was performed on a reversed-phase HPLC system with electrochemical detection (ED) using an internal standard. Validation of the analytical method showed that the system is well suited for pharmacokinetic studies on dogs. The assay was linear in the range 1-50 ng/ml. Inter-day and intra-day variability were between 6.43-18.15% C.V. and 1.57-5.53% C.V., respectively.