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A bright and photostable photoconvertible fluorescent protein

2009; Nature Portfolio; Volume: 6; Issue: 2 Linguagem: Inglês

10.1038/nmeth.1296

1548-7105

Sean McKinney, Christopher S. Murphy, Kristin L. Hazelwood, Michael W. Davidson, Loren L. Looger,

Photosynthetic Processes and Mechanisms

An improved version of the green-to-red photoconverting EosPF is presented. mEos2 is a functional fusion partner for many cellular proteins and retains the high localization precision of EosFP in super-resolution imaging. Also in this issue, Subach et al. present an inducible mCherry variant for super-resolution imaging. Photoconvertible fluorescent proteins are potential tools for investigating dynamic processes in living cells and for emerging super-resolution microscopy techniques. Unfortunately, most probes in this class are hampered by oligomerization, small photon budgets or poor photostability. Here we report an EosFP variant that functions well in a broad range of protein fusions for dynamic investigations, exhibits high photostability and preserves the ∼10-nm localization precision of its parent.