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Induction of partial limb regeneration in Rana pipiens by galvanic stimulation

1967; Wiley; Volume: 158; Issue: 1 Linguagem: Inglês

10.1002/ar.1091580110

1097-0185

Stephen D. Smith,

Silk-based biomaterials and applications

Abstract Forelimbs of small adult frogs ( Rana pipiens ) were amputated and the stumps implanted with silver‐platinum bimetallic rods insulated except at the tips. The silver end was at the wound surface in some cases. In others, the platinum end was distally placed. Other animals received separate implants of short pieces of bare silver and platinum wire, with the silver piece placed near the wound surface. Controls consisted of animals with either no implant or implants of partially insulated silver or platinum wire. Only one of the control animals (one with a silver implant) initiated regeneration. Regeneration was minimal with the separate implants (25%), more frequent with the platinum end of a bimetallic couple at the wound surface (70%), and most frequent (83.4%) with the silver end of a bimetallic couple at the wound surface. In three cases in the last named series, lobulated cartilages reminiscent of wrist and hand elements differentiated. The results are ascribed to direct stimulation of the cells, electrophoretic transfer of information‐bearing molecules, or stimulation of nerves.