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Evidence for the Non-Peptide Nature of Neutrophil-Derived Eosinophil Chemotactic Factor

1981; Karger Publishers; Volume: 65; Issue: 1 Linguagem: Inglês

10.1159/000232733

1423-0097

Beate M. Czarnetzki, R. E. Zimmermann,

Protease and Inhibitor Mechanisms

Neutrophil-derived low molecular weight eosinophil chemotactic factor (ECF_PMN) was examined by a number of methods to prove its peptide nature. Sephadex G-10 eluates of ECF_PMN were analyzed by paper chromatography and thin-layer chromatography, and peptides were separated on a peptide analyzer. Peptides isolated by these three different methods have no neutrophil or eosinophil chemotactic activity over a wide range of concentrations. Enzyme digestion studies supported these findings. Other physical and biological properties of ECF_PMN suggest that this material is most likely lipid in nature.