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Nanocomposites Based on PLLA and Multi Walled Carbon Nanotubes Support the Myogenic Differentiation of Murine Myoblast Cell Line

2013; Hindawi Publishing Corporation; Volume: 2013; Linguagem: Inglês

10.1155/2013/825912

2314-4696

Simona Montesano, Erlantz Lizundia, Francesco D’Angelo, Elena Fortunati, Samantha Mattioli, Francesco Morena, Ilaria Bicchi, Fabio Naro, Maurilio Sampaolesi, Jose‐Ramon Sarasua, J. M. Kenny, Aldo Orlacchio, I. Armentano, Sabata Martino,

Muscle Physiology and Disorders

We explored the effect of poly(L-lactic acid) (PLLA) containing various percentages (0.1, 0.5, 1, and 3 wt.%) of multi walled carbon nanotubes (MWCNTs) on the myogenic differentiation of C2C12 murine myoblast progenitor cells. We showed that all PLLA/MWCNTs nanocomposite materials support the myotubes formation more efficiently than neat PLLA as indicated by the high expression of the most significant myogenic markers: MyoD, Myosin Heavy Chain, dimension of myofibres, and fusion myogenic index. Interestingly, we note that both MyoD and myogenic fusion index levels were in the order 0.1 MWCNTs = 0.5 MWCNTs > 1 MWCNTs > 3 MWCNTs > neat PLLA, suggesting that the amount of MWCNTs influenced the cell differentiation.