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Cloning of Human and Mouse cDNAs Encoding Novel Zinc Finger Proteins Expressed in Cerebellum and Hippocampus

1997; Elsevier BV; Volume: 231; Issue: 2 Linguagem: Inglês

10.1006/bbrc.1996.6033

1090-2104

Koji Yasojima, Atsushi Tsujimura, Toshiki Mizuno, Yasufumi Shigeyoshi, J. Inazawa, Reiko Kikuno, Kei‐ichi Kuma, Kohsaku Ohkubo, Yohei Hosokawa, Yasuhiko Ibata, Tatsuo Abe, Takashi Miyata, Kenichi Matsubara, Kenji Nakajima, Tamotsu Hashimoto-Gotoh,

RNA and protein synthesis mechanisms

We identified a novel gene,kf-1,highly expressed in the normal cerebellum but not in the cerebral cortex, the expression of which could have been augmented in the cerebral cortex of a sporadic Alzheimer's disease patient. We cloned human and mouse entirekf-1cDNAs encoding conserved 79 kDa proteins containing a zinc-binding RING-H2 finger motif at the carboxy-terminus as found in acetylcholine receptor-associated protein (RAPsyn). The 3′-untranslated regions are highly conserved between human and mouse as to constitute a common mRNA secondary structure.In situhybridization analysis of mouse brain sections revealed strongkf-1expression in the cerebellum and hippocampus. We propose that KF-1 is involved in membranous protein-sorting apparatus similarly to RAPsyn. We mapped the humankf-1gene to 2p11.2.