A Functional Assay for Centromere-Associated Sister Chromatid Cohesion
1999; American Association for the Advancement of Science; Volume: 285; Issue: 5425 Linguagem: Inglês
10.1126/science.285.5425.254
ISSN1095-9203
AutoresPaul C. Megee, Douglas Koshland,
Tópico(s)Chromosomal and Genetic Variations
ResumoCohesion of sister chromatids occurs along the entire length of chromosomes, including the centromere where it plays essential roles in chromosome segregation. Here, minichromosomes in the budding yeast Saccharomyces cerevisiae are exploited to generate a functional assay for DNA sequences involved in cohesion. The centromeric DNA element CDEIII was found to be necessary but not sufficient for cohesion. This element was shown previously to be required for assembly of the kinetochore, the centromere-associated protein complex that attaches chromosomes to the spindle. These observations establish a link between centromere-proximal cohesion and kinetochore assembly.
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