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Quelques remarques complémentaires sur l'hydrolyse des triglycerides par la lipase pancréatique

1960; Elsevier BV; Volume: 43; Linguagem: Francês

10.1016/0006-3002(60)90412-1

1878-2434

Marieta Constantin, L. Paséro, P. Desnuelle,

Pancreatic function and diabetes

Some additional remarks on the hydrolysis of triglycerides by pancreaa lipase 1. In vitro lipolysis of triglycerides is impeded by difficulties due to the conflict between hydrolysis, on the one hand, and progressive inactivation of the lipase, on the other. The maximum rate of hydrolysis attainable consequently depends in the first place on the relation between the amounts of enzyme and substrate present. It is found that, when the initial proportion of the enzyme is increased, the lipolysis tends to become total. 2. However, even when lipolysis is ultimately total, considerable quantities of transitory partial glycerides are formed while lipolysis is going on. 3. The "activating" effect on lipolysis commonly attributed to calcium ions and bile salts is discussed. 4. Some consequences of these in vitro observations on the nature of the products absorbed by intestinal mucosa and on the mechanism of triglyceride resynthesis in this mucosa, are briefly considered. 1. La lipolyse in vitro des triglycérides est contrecarée par les difficultés croissantes de l'hydrolyse et l'inactivation progressive de la lipase. Le taux maximum d'enzyme par rapport au substrat. En augmentant les proportions initiales de la lipase, on constate que la lipolyse tend á devenir totale. 2. Même quand elle est finalement totale, la lipolyse engendre de falçon transitoire des quantités considérables de glycérides partiels. 3. Le rôle "activateur" communément attibué aux ions Ca++ et aux sels biliares pendant la lipolyse est discuté. 4. Quelques incidences possibles de ces observations in vitro sur la nature des produits absorbés par la muqueuse intestinale et sur le mécanisme de la resynthése des triglycérides dans cette muqueuse, sont brièvement envisagées.