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A novel RNA splicing-mediated gene silencing mechanism potential for genome evolution

2003; Elsevier BV; Volume: 310; Issue: 3 Linguagem: Inglês

10.1016/j.bbrc.2003.09.070

1090-2104

Shi-Lung Lin, Donald C. Chang, Da-Yu Wu, Shao‐Yao Ying,

RNA regulation and disease

Over 90% of the human genome consists of non-protein-coding regions. Introns constitute most of the non-coding regions located in precursor messenger RNAs (pre-mRNAs). During pre-mRNA maturation, the introns are excised out of mRNA and thought to be completely digested prior to translation. If the introns were merely metabolic "leavings," why would the genome hold such a large amount of extraneous genetic materials? Here we show a novel posttranscriptional gene silencing system identified within mammalian introns. By packaging human spliceosome-recognition sites along with an exonic insert into an artificial intron, we observed that the splicing and processing of such an exon-containing intron in either sense or antisense conformation produced equivalent gene silencing effects, while a palindromic hairpin insert containing both sense and antisense strands resulted in synergistic effects. These findings may explain how cells respond to the presence of transgenic introns that are homologous to pre-existing exons during genomic evolution.