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ULTRAFILTRABLE MAGNESIUM IN HYPERTHYROIDISM

1939; American Society for Clinical Investigation; Volume: 18; Issue: 5 Linguagem: Inglês

10.1172/jci101073

1558-8238

Louis J. Soffer, D. Alfred Dantes, Edward B. Grossman, Harry Sobotka, Mildred D. Jacobs,

Magnesium in Health and Disease

The following report is concerned with the study of magnesium metabolism in clinical and experimental hyperthyroidism.This first com- munication deals primarily with the ratio of diffusible magnesium to total serum magnesium in the hyperthyroid state.The general question of relationship of total serum magnesium to its ultrafiltrable fraction in 8 normal individuals has been discussed by Watchorn and McCance (1).They found that normally approximately 25 per cent of the total serum magnesium is non-diffusible.These authors suggest that there are probably 2 factors which play a part in determining the amount of ionizable magnesium.The bound magnesium may be in combination either with the protein in the serum or with the serum phosphatides.These acids, which behave similarly to protein, are pres- ent in adequate quantities to bind considerable amounts of magnesium.Brull (2) and Scholtz (3) also believe that both calcium and magnesium form colloidal phosphate complexes. METHODThe total serum magnesium was determined by the method of Briggs (4).The serum proteins were precipitated with trichloracetic acid.It was found that little, if any, magnesium was carried down with the protein flocculum.To 10 cc. of protein free filtrate were added 1 cc. of 20 per cent sodium acetate, 6 to 8 drops of 0.016 per cent bromcresol green, and 1 cc. of 4 per cent ammonium oxalate.The pH of the solution was adjusted to 5.0 by addition of ammonium hydrox- ide.The mixture was allowed to stand overnight, and the precipitated calcium oxalate then separated by centrifugation.To the decanted supernatant fluid and washings were added 1 cc. of 2 per cent potassium dihydrogen phosphate,