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Sensitive fused-silica capillary gas chromatographic assay using electron-capture detection for indomethacin in ovine fetal fluids

1995; Elsevier BV; Volume: 674; Issue: 1 Linguagem: Inglês

10.1016/0378-4347(95)00291-x

1872-812X

Rajesh Krishna, K. Wayne Riggs, Martin Walker, Eddie Kwan, Dan W. Rurak,

Inflammatory mediators and NSAID effects

A sensitive gas chromatographic (GC) method with electron-capture detection (ECD) has been developed to quantitate indomethacin (IND) in plasma, urine, amniotic, and tracheal fluids obtained from the pregnant sheep model. IND and the internal standard, α-methylindomethacin (α-Me-IND) are extracted by a simple liquid-liquid extraction procedure using ethyl acetate and derivatized with N-methyl-N-(tert.-butyldimethylsilyl)trifluoroacetamide (MTBSTFA) at 60°C for 50 min. The limit of quantitation (LOQ) is 1 ng/ml with a C.V. 10. Recoveries from all fluids were greater than 80%. Calibration curves were linear over the range of 1–32 ng/ml with a coefficient of determination (r2) > 0.999. Inter- and itra-day coefficients of variation were <10% at concentrations of 2–32 ng/ml, and <20% at the LOQ. Applicability of the developed method is demonstrated for a pharmacokinetic study of IND samples collected following long-term infusion of IND in a chronically instrumented ovine fetus.