Changes in Levels of α-Amylase Components in Barley Tissues during Germination and Early Seedling Growth
1984; Oxford University Press; Volume: 75; Issue: 1 Linguagem: Inglês
10.1104/pp.75.1.203
ISSN1532-2548
AutoresA. W. MacGregor, F. Helen MacDougall, Christiane Mayer, J. Daussant,
Tópico(s)Enzyme Production and Characterization
ResumoKernels of Klages barley (Hordeum vulgare L.) were germinated for 1 to 4 days on moist sand at 18 degrees C. Representative kernels from each time period were dissected to give the following fractions: scutellum, subscutellar endosperm, aleurone-scutellum interface, remaining aleurone, subaleurone endosperm, and core endosperm. These tissues were analyzed for alpha-amylase components by isoelectric focusing and rocket-line immunoelectrophoresis. Although aleurone and scutellar tissues appeared to synthesize the same alpha-amylase components, enzyme was detected first in the scutellum. A larger proportion of scutellar alpha-amylase was excreted into the endosperm compared to aleurone synthesized alpha-amylase. Aleurone cells appeared to synthesize appreciably more alpha-amylase than did scutellar tissue.
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