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Specific Gene Repression by CRISPRi System Transferred through Bacterial Conjugation

2014; American Chemical Society; Volume: 3; Issue: 12 Linguagem: Inglês

10.1021/sb500036q

2161-5063

Weiyue Ji, Derrick Lee, Eric B. Wong, Priyanka Dadlani, David Dinh, Verna Huang, Kendall Kearns, Sherry Teng, Susan Chen, John Haliburton, Graham Heimberg, Benjamin M. Heineike, Anusuya Ramasubramanian, Thomas Stevens, Kara J. Helmke, Veronica Zepeda, Lei S. Qi, Wendell A. Lim,

Gene Regulatory Network Analysis

In microbial communities, bacterial populations are commonly controlled using indiscriminate, broad range antibiotics. There are few ways to target specific strains effectively without disrupting the entire microbiome and local environment. Here, we use conjugation, a natural DNA horizontal transfer process among bacterial species, to deliver an engineered CRISPR interference (CRISPRi) system for targeting specific genes in recipient Escherichia coli cells. We show that delivery of the CRISPRi system is successful and can specifically repress a reporter gene in recipient cells, thereby establishing a new tool for gene regulation across bacterial cells and potentially for bacterial population control.