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In vitro induction of interferon-like activity from rainbow trout leucocytes stimulated by Egtved virus

1993; Elsevier BV; Volume: 3; Issue: 5 Linguagem: Inglês

10.1006/fsim.1993.1037

1095-9947

Claire Rogel Gaillard, Stéfan Chilmonczyk, P. de Kinkelin,

Viral Infections and Vectors

Interferon (IFN)-like activity was induced in vitro from blood and kidney leucocytes by infectious or inactivated Egtved virus (VHSV) serotype 1. The IFN activity in the cell culture medium was determined by spectrophotometric assessment of its ability to protect RTG-2 cells against infectious pancreatic necrosis virus infection (virus strain VR299). Characteristics of this protective activity were consistent with those described for IFN α/β of mammals. IFN synthesis was induced by either infectious or inactivated virus. The yield of IFN depended on cell origin (blood or kidney) and donor, while temperature elevation enhanced the kinetics of this synthesis. Induction was abolished by treatment of glutaraldehyde-fixed VHSV-infected EPC cells with a neutralising anti-G protein specific monoclonal antibody (MAb), whereas MAbs specific or other viral proteins had no effect. This suggests that the induction of IFN synthesis in trout could be independent, in part, from Egtved virus multiplication and thus could be triggered by contact between target fish cells and one of the virus surface components. Such a mechanism, acting immediately after the virus entry into the fish, might be involved in the virulence. Passive transfer of IFN-like activity to trout alevins resulted in protection against water borne challenge with Egtved virus. The present results encourage further investigations on the role played by IFN in the pathogenesis of viral haemorrhagic septicaemia.