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Isolation and characterization of globulin from seeds of Amaranthus hypochondriacus L.

1985; Oxford University Press; Volume: 49; Issue: 5 Linguagem: Inglês

10.1271/bbb1961.49.1453

1881-1280

Yôtarô Konishi, Yuko Fumita, Kayoko Ikeda, Kazutoshi Okuno, Hidetsugu FUWA,

Food composition and properties

Seeds of grain amaranths contain a high amount (about 60% of total nitrogen) of albumin and globulin and a trace amount of prolamin. From salt-soluble extracts of A. hypochondriacus seeds, a globulin (440, 000 in apparent molecular weight and s020, w= 12-7) was purified by Sepharose 6B gel and DEAE-cellulose column chromatographies. The protein comprised at least four kinds of subunits whose molecular weights were 36, 000, 32, 000, 20, 000 and 18, 000, respectively. The amino acid composition of the globulin was almost similar to those of soybean and oat globulins.