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Determination of vincristine in infant plasma by liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy

2006; Elsevier BV; Volume: 41; Issue: 2 Linguagem: Inglês

10.1016/j.jpba.2005.11.039

1873-264X

Mark S. Schmidt, Rong Huang, Robert J. Classon, Daryl J. Murry,

Antibiotics Pharmacokinetics and Efficacy

An LC-MS method using APCI has been developed and validated for the determination of the anticancer drug vincristine in human plasma, using vinblastine as internal standard. Following solid-phase extraction (SPE) of the sample, the lower limit of quantitation (LLOQ) was 0.18 ng/ml, the lower limit of detection was 0.09 ng/ml, and the linear calibration range was 0.18–180 ng/ml. This method has been used to measure plasma concentrations of vincristine from 0.08 to 24 h post bolus in 29 infants as part of a pharmacokinetic study. Concentrations of vincristine at 24 h were 0.2–1.36 ng/ml.