Produção Nacional
Revisado por pares
Pretreatment With L‐Citrulline Positively Affects the Mucosal Architecture and Permeability of the Small Intestine in a Murine Mucositis Model
2015; Wiley; Volume: 40; Issue: 2 Linguagem: Inglês
10.1177/0148607114567508
ISSN1941-2444
AutoresMaísa Mota Antunes, Paola Caroline Lacerda Leocádio, Lílian Gonçalves Teixeira, Alda Jusceline Leonel, Denise Carmona Cara, Gustavo Batista Menezes, Simone de Vasconcelos Generoso, Valbert Nascimento Cardoso, Jacqueline I. Alvarez‐Leite, María Isabel Toulson Davisson Correia,
Tópico(s)Neutropenia and Cancer Infections
ResumoBackground: Mucositis is a common complication in patients undergoing radiotherapy and chemotherapy. It is associated with pain, poor quality of life, and malnutrition, leading to an increased number of hospital admissions and prolonged hospitalization. The use of immunonutrients may be an alternative treatment option, which may help to improve patient outcome. Objective : Here we assessed the impact of L‐citrulline (CIT) on a murine model of 5‐fluorouracil (5FU)–induced mucositis. Methods : Swiss male mice were randomized into 4 groups: control, CIT, 5FU, and 5FU+CIT. Mice were fed with commercial chow and supplemented with an oral solution of alanine (control and 5FU groups) or CIT (CIT and 5FU+CIT groups). On the seventh day, mice received intraperitoneal phosphate‐buffered saline or 5FU (200 mg/kg, single dose) to induce mucositis. On the 10th day, mice were euthanized, and the blood and small intestines were harvested. Body weight, morphology, histopathology score (hematoxylin and eosin) of the small intestine (from 0–12), myeloperoxidase activity, oxidative stress level, and intestinal permeability were assessed. Results : We observed significant weight loss after the administration of 5FU in both treated and control animals. CIT administration contributed to a partial recovery of the mucosal architecture as well as an intermediate reduction of the histopathologic score, and functional intestinal permeability was partially rescued. Conclusions : CIT administration attenuated 5FU‐mediated damage to the mucosal architecture of the small intestine, decreasing the size of the injured areas and promoting decreased intestinal permeability.
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