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Selective Isotope Labelling of Leucine Residues by Using α‐Ketoacid Precursor Compounds

2013; Wiley; Volume: 14; Issue: 7 Linguagem: Inglês

10.1002/cbic.201200737

1439-7633

Roman Lichtenecker, Nicolas Coudevylle, Robert Konrat, Walther Schmid,

Microbial Metabolic Engineering and Bioproduction

You can have one without the other: A new metabolic precursor compound can selectively introduce (13)C and (2)H patterns at leucine residues in proteins in cell-based expression systems without interfering with valine metabolism. The protocol provides selectively labelled macromolecules well suited for probing structure and dynamics in high-molecular-weight proteins by NMR spectroscopy.