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Effect of oral consumption of probiotic Lactobacillus planatarum P-8 on fecal microbiota, SIgA, SCFAs, and TBAs of adults of different ages

2013; Elsevier BV; Volume: 30; Issue: 7-8 Linguagem: Inglês

10.1016/j.nut.2013.11.018

1873-1244

Lifeng Wang, Jiachao Zhang, Zhuang Guo, Lai‐Yu Kwok, Chen Ma, Wenyi Zhang, Qiang Lv, Weiqiang Huang, Heping Zhang,

Clostridium difficile and Clostridium perfringens research

Probiotics may improve intestinal health by modulating intestinal microbiota. However, the age-related variation in response to probiotic consumption is understudied. The aim of this study was to determine the effect of oral consumption of Lactobacillus plantarum P-8 (Lp-8) on human intestinal microflora, secretary immunoglobulin A (SIgA), total bile acids (TBAs), and short-chain fatty acids (SCFAs) of different aged adults. Thirty-three recruited individuals, including young (mean age 26 y), middle-aged (mean age 51 y), and elderly (mean age 76 y) volunteers, were given a single daily oral dose of Lp-8 (6 × 1010 colony forming units) for 4 wk. Fecal samples were collected before starting, during, and after stopping Lp-8 intake. Polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis, quantitative PCR, high-performance liquid chromatography, and enzyme-linked immunosorbent assay were used to detect for fecal microflora, SIgA, TBAs, and SCFAs. Results showed an increase in Bifidobacterium (P < 0.05) and other beneficial bacteria, whereas Desulfovibrio (P < 0.05) and other opportunistic pathogens decreased after taking Lp-8 for 4 wk. Lp-8 consumption also affected fecal levels of SIgA, TBAs, and SCFAs. Lp-8 administration could improve human gastrointestinal health. However, some of these effects were transient and gradually disappeared once the intervention was discontinued. Moreover, the extent of these desirable effects was age-related.