Phase separation in dextran/locust bean gum mixtures
1995; Elsevier BV; Volume: 28; Issue: 4 Linguagem: Inglês
10.1016/0144-8617(95)00090-9
ISSN1879-1344
AutoresCatherine Garnier, C Schorsch, J.L. Doublier,
Tópico(s)Proteins in Food Systems
ResumoDextran/locust bean gum (LBG) mixtures have been prepared and investigated with respect to their phase separation behaviour. These systems exhibited phase separation at 20 °C, the upper phase, itself biphasic, being enriched with locust bean gum but also containing dextran, whereas the lower phase contained only dextran. This lower phase was a liquid. The upper phase, which did not flow, was characterized by means of rheological dynamic measurements. Clearly, its behaviour was typical of a gel, the three-dimensional structure of which can be ascribed to self-association of LBG chains owing to the very high concentration of the galactomannan in this upper phase. The self-association of the galactomannan was confirmed by fluorescence microscopy carried out on mixtures containing fluorescein isothiocyanate (FITC)-labelled dextran. The rheological behaviour of a concentrated LBG solution was also investigated as a function of time, clearly showing progressive formation of a weak gel structure.
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