Transformation of Rhododendron spp. using Agrobacterium tumefaciens with a GUS-intron chimeric gene
1997; Elsevier BV; Volume: 122; Issue: 2 Linguagem: Inglês
10.1016/s0168-9452(96)04544-x
ISSN1873-2259
AutoresD. Pavingerová, J. Břı́za, Karel Kodýtek, H. Niedermeierová,
Tópico(s)CRISPR and Genetic Engineering
ResumoThe five Rhododendron cultivars, 'America', 'Catawbiense grandiflorum roseum', 'Madame Carvalho', 'Mars' and 'Nova Zembla' were used for transformation by Agrobacterium tumefaciens carrying T-DNA with the gusA gene encoding β-glucuronidase (GUS) gene and the neomycin phosphotransferase II gene as a selectable marker gene. The GUS reporter gene was successfully transferred into all five cultivars as indicated by fluorimetric staining, polymerase chain reaction (PCR) and Southern blot analysis. Some primary transformants appeared to be chimeric as both GUS expression and GUS nucleotide sequences were lost during vegetative propagation.
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