Direct Electrochemistry of Cholesterol Oxidase Immobilized on a Conducting Polymer: Application for a Cholesterol Biosensor
2009; Wiley; Volume: 22; Issue: 1 Linguagem: Inglês
10.1002/elan.200900363
ISSN1521-4109
AutoresAdel A. Abdelwahab, Mi‐Sook Won, Yoon‐Bo Shim,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoAbstract Direct electrochemistry of cholesterol oxidase (ChOx) immobilized on the conductive poly‐3′,4′‐diamine‐2,2′,5′,2″‐terthiophene (PDATT) was achieved and used to create a cholesterol biosensor. A well‐defined redox peak was observed, corresponding to the direct electron transfer of the FAD/FADH 2 of ChOx, and the rate constant ( k s ) was determined to be 0.75 s −1 . Glutathione (GSH) covalently bonded with PDATT was used as a matrix for conjugating AuNPs, ChOx, and MP, simultaneously. MP co‐immobilized with ChOx on the AuNPs‐GSH/PDATT exhibited an excellent amperometric response to cholesterol. The dynamic range was from 10 to 130 μM with a detection limit of 0.3±0.04 μM.
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