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Transcription of human 5S rRNA genes is influenced by an upstream DNA sequence

1993; Oxford University Press; Volume: 21; Issue: 16 Linguagem: Inglês

10.1093/nar/21.16.3631

1362-4962

Jens Nederby Nielsen, Charlotte Hallenberg, Sune Frederiksen, Pernille Dissing Sørensen, Bodil Lomholt,

RNA modifications and cancer

Six human 5S rRNA genes and gene variants and one pseudogene have been sequenced. The six genes/variants were transcribed In a HeLa cell extract with about equal efficiency. Three genes contain the Sp1 binding sequence GGGCGG in position -43 to -38 and three genes contain the Sp1 like sequence GGGCCG In this position. The six genes contain furthermore one Sp1 binding site in a position about -245 and one ATF recognition site in a position about -202. A 12 bp sequence (GGCTCTTGGGGC) found in position -32 to -21 strongly influenced the transcriptlonal efficiency in vitro . This 12-mer, designated the D box, has also been found upstream a 5S rRNA gene from hamster and mouse. Removal of the Sp1 binding sites had no effect on the transcription in vitro whereas the transcriptlonal efficiency decreased to 10% If the D vox was removed from the human 5S rRNA gene.