Hydroxamic acid content in undifferentiated and differentiated tissues of wheat
1991; Elsevier BV; Volume: 30; Issue: 10 Linguagem: Inglês
10.1016/0031-9422(91)83193-o
ISSN1873-3700
AutoresGustavo E. Zúñiga, Francisca Massardo,
Tópico(s)Plant Parasitism and Resistance
ResumoHydroxamic acid content in wheat tissues was studied by HPLC. Undifferentiated tissues such as callus or meristem produce only the aglucones, 2,4-dihydroxy-1,4-benzoxazin-3-one (DIBOA) and 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA). DIMBOA β-glucosidase activity was not detectable in these tissues. In seedlings, DIBOA-glucoside first appeared and then DIMBOA-glucoside. In seven-day-old seedlings a significant amount of aglucone was also detected, although the β-glucosidase activity was inhibited by liquid nitrogen and methanol. The results suggest that hydroxamic acids are produced by undifferentiated tissues as aglucones; later glucosylation gives rise to the glucosides normally detected in differentiated shoots or roots.
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