Characteristics of β-adrenergic receptors in bovine corneal epithelium: Comparison of fresh tissue and cultured cells
1984; Elsevier BV; Volume: 121; Issue: 2 Linguagem: Inglês
10.1016/0006-291x(84)90233-x
ISSN1090-2104
AutoresRonald J. Walkenbach, Scott R. Gibbs, David B. Bylund, Wan-Tsih H. Chao,
Tópico(s)Receptor Mechanisms and Signaling
ResumoThe characteristics of the β-adrenergic receptors in homogenates of fresh tissue and cultured bovine corneal epithelium were compared using [3H]dihydroalprenolol. High affinity, specific binding sites were observed in both preparations. Fresh tissue exhibited a higher binding site density (165 fmol/mg protein) than did cells in culture (57 fmol/mg protein). Studies with various β-adrenergic agonists and antagonists indicated that binding characteristics were typical of β-adrenergic receptors, predominantly of the β2 subtype. These results demonstrate that β-adrenergic receptors exist in both fresh and cultured bovine corneal epithelium and that these receptors are qualitatively and quantitatively similar.
Referência(s)