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In Vitro Effects of Human Umbilical Cord Perivascular Cells Conditioned Media on Populations of Dopaminergic Neurons

1900; Frontiers Media; Volume: 3; Linguagem: Inglês

10.3389/conf.neuro.01.2009.11.091

1662-4548

António J. Salgado,

Tissue Engineering and Regenerative Medicine

Event Abstract Back to Event In Vitro Effects of Human Umbilical Cord Perivascular Cells Conditioned Media on Populations of Dopaminergic Neurons G. Baltazar1, F. Campos1, C. P. Fonseca1, A. C. Cristovão1, J. S. Fraga2*, N. M. Neves3, 4, R. L. Reis3, 4, N. Sousa2 and A. J. Salgado2 1 University of Beira Interior, Research Center in Health Sciences- CICS, Portugal 2 University of Minho, Life and Health Sciences Research Institute (ICVS), School of Health Sciences, Portugal 3 Department of Polymer Engineering, University o Minho, 3B’s Research Group – Biomaterials, Biodegrables and Biomimetics, Portugal 4 IBB- Institute for Biotechnology and Bioengineering, PT Government Associated Laboratory, Portugal Parkinson’s disease (PD) is a neurodegenerative condition in which the populations of dopaminergic neurons are highly affected, leading to consequences that have a major impact on the patients’ life-quality. It has been recently shown that mesenchymal progenitors isolated from the Wharton jelly of the umbilical cord ameliorate the condition of parkisonian rats upon transplantation. It has been hypothesized that these actions are mainly mediated through neurotrophic factors secreted by the cord transplanted cells. However, to our knowledge, this fact is yet to be demonstrated. Therefore, the objective of the present work was the determine the effect of media conditioned obtained from a population of mesenchymal progenitors known as Human Umbilical Cord Perivascular cells (HUCPVCs) on mesencephalic neurons and in particular in the dopaminergic population.HUCPVCs were isolated from the primitive connective tissue present in umbilical cord and further cultured for 3 days at 4000cells/cm2. On day 3 the medium was replaced and 24h, 48h, 72h and 96h thereafter HUCPVCs conditioned media (CM) was collected. This CM was applied onto primary ventral mesencephalic cultures prepared from E15-E16 Wistar rat embryos. Neuronal cells were kept in culture for five days in neurobasal medium supplemented with B27 before being briefly washed and further incubated for 72h with the CM or with medium not subjected to the conditioning process (Control). After this incubation period cells were processed for MAP2 immunocytochemistry and for analysis of tyrosine hydroxylase (TH) expression by Western blot. The effect of the CM on the overall neuronal viability was also examined through the MTT test.Results revealed that CM increased the metabolic viability of the cultures when compared to controls, with a 34% increase in MTT+ reduction for cells treated with 48h CM. Moreover, TH expression was also increased by 45, 55 and 51% in cultures exposed to media conditioned for 24, 48 and 96h, respectively. The protective effect of the CM does not seem to be specific to the dopaminergic neurons since an overall increase on both the number of MAP-2+ cells and TH+ neurons was observed for 24h and 72h CM. Conference: 11th Meeting of the Portuguese Society for Neuroscience, Braga, Portugal, 4 Jun - 6 Jun, 2009. Presentation Type: Poster Presentation Topic: Abstracts Citation: Baltazar G, Campos F, Fonseca CP, Cristovão AC, Fraga JS, Neves NM, Reis RL, Sousa N and Salgado AJ (1900). In Vitro Effects of Human Umbilical Cord Perivascular Cells Conditioned Media on Populations of Dopaminergic Neurons. Front. Neurosci. Conference Abstract: 11th Meeting of the Portuguese Society for Neuroscience. doi: 10.3389/conf.neuro.01.2009.11.091 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 01 Jan 1900; Published Online: 01 Jan 1900. * Correspondence: J. S Fraga, University of Minho, Life and Health Sciences Research Institute (ICVS), School of Health Sciences, Braga, Portugal, joanafraga@ecsaude.uminho.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers G. Baltazar F. Campos C. P Fonseca A. C Cristovão J. S Fraga N. M Neves R. L Reis N. Sousa A. J Salgado Google G. Baltazar F. Campos C. P Fonseca A. C Cristovão J. S Fraga N. M Neves R. L Reis N. Sousa A. J Salgado Google Scholar G. Baltazar F. Campos C. P Fonseca A. C Cristovão J. S Fraga N. M Neves R. L Reis N. Sousa A. J Salgado PubMed G. Baltazar F. Campos C. P Fonseca A. C Cristovão J. S Fraga N. M Neves R. L Reis N. Sousa A. J Salgado Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.