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DNA Cell Cycle Studies In Uveal Melanoma

1989; Elsevier BV; Volume: 107; Issue: 1 Linguagem: Inglês

10.1016/0002-9394(89)90817-9

1879-1891

Devron H. Char, Kim Huhta, Frederic M. Waldman,

Cutaneous Melanoma Detection and Management

We histologically studied uveal melanomas treated with surgery only (enucleation or ciliochoroidectomy), low-dose (20 Gy) preenucleation radiation followed by enucleation, or enucleated melanomas after high-dose (50 to 80 Gy) charged-particle beam therapy. There was significantly less bromodeoxyuridine uptake in irradiated vs nonradiated melanomas (P < .0001). Similarly, tissue culture growth of irradiated tumors was significantly less (P < .007). These data demonstrate destruction of reproductive integrity of helium ion-irradiated melanomas. The incorporation of bromodeoxyuridine and fine needle aspiration biopsy techniques may be useful in the delineation of successfully irradiated tumors from tumors with apparent growth secondary to radiation vasculopathy. We histologically studied uveal melanomas treated with surgery only (enucleation or ciliochoroidectomy), low-dose (20 Gy) preenucleation radiation followed by enucleation, or enucleated melanomas after high-dose (50 to 80 Gy) charged-particle beam therapy. There was significantly less bromodeoxyuridine uptake in irradiated vs nonradiated melanomas (P < .0001). Similarly, tissue culture growth of irradiated tumors was significantly less (P < .007). These data demonstrate destruction of reproductive integrity of helium ion-irradiated melanomas. The incorporation of bromodeoxyuridine and fine needle aspiration biopsy techniques may be useful in the delineation of successfully irradiated tumors from tumors with apparent growth secondary to radiation vasculopathy.