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CHANGES IN LIVER GLYCOGEN STUDIED BY THE NEEDLE ASPIRATION TECHNIC IN PATIENTS WITH DIABETIC KETOSIS. WITH A METHOD FOR THE ESTIMATION OF GLYCOGEN FROM HISTOLOGIC PREPARATIONS 1

1949; American Society for Clinical Investigation; Volume: 28; Issue: 5 Pt 2 Linguagem: Inglês

10.1172/jci102155

1558-8238

Philip K. Bondy, Walter H. Sheldon, Lillian D. Evans,

Diabetes and associated disorders

Previous attempts to estimate the glycogen content of the human liver were based on deter- minations performed on material obtained by op- erative biopsy or from postmortem specimens.Observations on animals have shown that these procedures may introduce an important error through the effect of the commonly used anesthetic agents, or of trauma, before the specimen is ob- tained.The development of a technic for the re- moval of small specimens of liver by aspiration biopsy is well adapted to the study of glycogen metabolism, for it avoids the uncertain effects of premedication, general anesthesia and the trauma of operation.By this method serial biopsies of the liver have been obtained in a study of patients under treatment for diabetic acidosis. METHODSLiver tissue was obtained by needle biopsy, using the Vim-Silverman needle, under local procaine infiltration.With practice, it was possible to obtain an adequate specimen in less than three seconds from the time of insertion of the needle into the substance of the liver.Speed was desirable, since patients in severe acidosis were unable to hold their breath.The specimens were immediately fixed in iced absolute alcohol or Rossman's fixative.Plasma carbon dioxide combining powers were deter- mined by the method of Van Slyke and Neill (1).The Folin-Wu method was used for the determination of blood glucose (2). THE TECHNIC FOR THE ESTIMATION OF HEPATIC GLYCOGENAt first, the glycogen concentration was determined by the chemical method of Good, Kramer and Somogyi (3).This technic was unsatisfactory, however, since without histologic examination it was impossible to be