Use of positive ion fast atom bombardment mass spectrometry for rapid identification of a bile alcohol glucuronide isolated from cerebrotendinous xanthomatosis patients
1990; Elsevier BV; Volume: 55; Issue: 2 Linguagem: Inglês
10.1016/0039-128x(90)90028-a
ISSN1878-5867
AutoresB. Dayal, G Salen, G. Stephen Tint, Sarah Shefer, Siegfried Benz,
Tópico(s)Analytical Chemistry and Chromatography
ResumoThe identification of a major biliary and plasma bile alcohol glucuronide, 5β-cholestane-3α,7α,12α,25-tetrol-3-O-β-d-glucuronide, present in cerebrotendinous xanthomatosis (CTX) patients, was investigated by positive ion fast atom bombardment mass spectrometry (FAB-MS). The spectrum was characterised by abundant ions formed by attachment of a proton, [M + H]+, or of alkali ions, [M + Na]+ and [M + 39K]+, to the glucuronide salt. These ions allowed an unambiguous deduction of the molecular weight of the sample. It is suggested that FAB-MS could be used m the rapid diagnosis of CTX. (Steroids 55:74–78, 1990)
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