Produção Nacional
Revisado por pares
Phytochemical Prospection and Modulation of Antibiotic Activity In Vitro by Lippia origanoides H.B.K. in Methicillin Resistant Staphylococcus aureus
2014; Hindawi Publishing Corporation; Volume: 2014; Linguagem: Inglês
10.1155/2014/305610
ISSN2314-6141
AutoresHumberto Medeiros Barreto, Filipe Cerqueira Fontinele, Aldeídia Pereira de Oliveira, Daniel Dias Rufino Arcanjo, Bernadete Helena Cavalcanti dos Santos, Aislan Pereira Lira de Abreu, Henrique Douglas Melo Coutinho, Romézio Alves Carvalho da Silva, Taciana Oliveira de Sousa, Maria das Graças Freire de Medeiros, Antônia Maria das Graças Lopes Citó, José Arimatéia Dantas Lopes,
Tópico(s)Plant chemical constituents analysis
ResumoThe Lippia origanoides H.B.K. ethanol extract (LOEE) and hexane (LOHEX), dichloromethane (LODCM), and ethyl acetate (LOEA) fractions were tested for their antimicrobial activity alone or in combination with antibiotics against a methicillin resistant Staphylococcus aureus (MRSA) strain. The natural products did not show antimicrobial activity against multidrug resistant strain at the clinically significant concentrations tested. However, a modulatory effect in the antibacterial activity of the neomycin and amikacin was verified when LOEE, LOHEX and LODCM were added to the growth medium at subinhibitory concentrations. A similar modulation was found when the natural products were changed for chlorpromazine, an inhibitor of bacterial efflux pumps, suggesting the involvement of resistance mediated by efflux system in the MRSA tested. The fractions LOHEX and LODCM showed a modulatory activity bigger than their majority compounds (carvacrol, thymol, and naringenin), indicating that this activity is not due to their majority compounds only, but it is probably due to a synergism between their chemical components. These results indicate that L. origanoides H.B.K. can be a source of phytochemicals able to modify the phenotype of resistance to aminoglycosides in MRSA.
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