Adenosine-dependent enhancement by methylxanthines of excitatory synaptic transmission in hippocampus of rats
1992; Elsevier BV; Volume: 135; Issue: 1 Linguagem: Inglês
10.1016/0304-3940(92)90124-p
ISSN1872-7972
AutoresOlga Garaschuk, Yury Kovalchuk, Oleg Krishtal,
Tópico(s)Photoreceptor and optogenetics research
ResumoWhole-cell patch-clamp was used to investigate synaptic transmission in hippocampal slices. Excitatory post-synaptic currents (EPSCs) were facilitated by low (≤ 1 μM) adenosine (Ado) concentrations, while high concentrations had well-known inhibitory effects on the EPSC. When added on the background of preapplied Ado, methylaxanthines caused a large potentiation of EPSCs. At saturation, the enhanced EPSC could exceed the control almost by an order of magnitude. Pertussis toxin strongly impaired the ability of Ado to block EPSCs but did not augment the facilitatory effect. The two components of the EPSC mediated by N-methyl-d-aspartate (NMDA) and non-NMDA receptors were facilitated simultaneously and in equal proportions.
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