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Antibodies against Tetrahymena 14-nm filament-forming protein recognize the replication band in Euplotes

1991; Elsevier BV; Volume: 193; Issue: 1 Linguagem: Inglês

10.1016/0014-4827(91)90554-8

1090-2422

Osamu Numata, T. Tomiyoshi, Y. KURASAWA, Fu Zhen-Xing, Mihoko Takahashi, Toshikazu Kosaka, Joe Chiba, Yoshio Watanabe,

DNA Repair Mechanisms

Tetrahymena 14-nm filament-forming protein (49K protein) is a structural protein which is involved in activity of the pronuclei during conjugation (O. Numata, T. Sugai, and Y. Watanabe (1985) Nature (London) 314, 192-194). Using monoclonal and polyclonal antibodies, we here demonstrate the presence of a cross-reactive protein (CRP-49) within the macronuclear replication bands of Euplotes harpa and E. eurystomus which is recognized by anti-49K protein antibodies. Immunoblotting reveals that both monoclonal and polyclonal antibodies cross-react to a protein with an apparent molecular mass of 50 kDa in an E. harpa cell extract and to a protein of 49 kDa in a macronuclear extract of E. eurystomus. The antibodies used in this study have no effect upon in vitro DNA synthesis in the replication band of E. eurystomus.