G-TRACE: rapid Gal4-based cell lineage analysis in Drosophila
2009; Nature Portfolio; Volume: 6; Issue: 8 Linguagem: Inglês
10.1038/nmeth.1356
ISSN1548-7105
AutoresCory J. Evans, John M. Olson, Kathy Ngo, Eunha Kim, Noemi E Lee, Edward Kuoy, Alexander N. Patananan, Daniel Sitz, Phuong‐Thao Tran, Minh-Tu Do, Kevin Yackle, Albert Cespedes, Volker Hartenstein, Gerald B. Call, Utpal Banerjee,
Tópico(s)Viral Infectious Diseases and Gene Expression in Insects
ResumoA Gal4-based system in Drosophila reports on gene expression at a given developmental stage combined with lineage information on expression at earlier developmental stages. We combined Gal4-UAS and the FLP recombinase–FRT and fluorescent reporters to generate cell clones that provide spatial, temporal and genetic information about the origins of individual cells in Drosophila melanogaster. We named this combination the Gal4 technique for real-time and clonal expression (G-TRACE). The approach should allow for screening and the identification of real-time and lineage-traced expression patterns on a genomic scale.
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