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BIBTEX RIS

Gold, Poly(β-amino ester) Nanoparticles for Small Interfering RNA Delivery

2009; American Chemical Society; Volume: 9; Issue: 6 Linguagem: Inglês

10.1021/nl9009793

ISSN

1530-6992

Autores

Jae‐Seung Lee, Jordan J. Green, Kevin T. Love, Joel Sunshine, Róbert Langer, Daniel G. Anderson,

Tópico(s)

Nanopore and Nanochannel Transport Studies

Resumo

The safe and effective delivery of RNA therapeutics remains the major barrier to their broad clinical application. Here we develop a new nanoparticulate delivery system based on inorganic particles and biodegradable polycations. First, gold nanoparticles were modified with the hydrophilic polymer poly(ethylene glycol) (PEG), and then small interfering RNA (siRNA) was conjugated to the nanoparticles via biodegradable disulfide linkages, with ∼30 strands of siRNA per nanoparticle. The particles were then coated with a library of end-modified poly(β-amino ester)s (PBAEs), previously identified as capable of facilitating intracellular DNA delivery. Nanoparticulate formulations developed here facilitate high levels of in vitro siRNA delivery, facilitating delivery as good or better than the commercially available lipid reagent, Lipofectamine 2000.

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