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Mobilization and transfer of Azospirillum lipoferum plasmid by the Tn 5 -Mob transposon into a plasmid-free Agrobacterium tumefaciens strain

1988; Canadian Science Publishing; Volume: 34; Issue: 12 Linguagem: Inglês

10.1139/m88-239

1480-3275

René Bally, Alain Givaudan,

Legume Nitrogen Fixing Symbiosis

Azospirillum lipoferum 4B harbors five cryptic plasmids. Several suicide plasmids were used to transfer Tn5-Mob to A. lipoferum 4B. Tn5-Mob insertion mutations of this strain could be obtained at frequencies of 10 −8 –10 −7 per recipient cell. One hundred Tn5-Mob A. lipoferum 4B mutants were used in bacterial matings with a plasmid-free Agrobacterium tumefaciens recipient strain. This is the first report of mobilization, transfer, and replication of an Azospirillum plasmid in Agrobacterium tumefaciens. One transconjugant was found which had lost an indigenous plasmid.