Cloning of chromosomal β-lactamase genes from Yersinia enterocolitica
1991; Microbiology Society; Volume: 137; Issue: 1 Linguagem: Inglês
10.1099/00221287-137-1-141
ISSN1465-2080
AutoresAsunción Seoane, Jorge M. Lobo,
Tópico(s)Botanical Research and Chemistry
ResumoSummary: Two β-lactamase genes present in the chromosome of Yersinia enterocolitica have been cloned individually into the plasmid pACY184 and expressed in Escherichia coli . The gene for broad-spectrum β-lactamase I (‘A’) was cloned from a strain belonging to the O:3 serotype, and the gene for (cephalosporinase) β-lactomase II (‘B’) was cloned from a strain of the O:5b serotype. The properties of the β-lactamases expressed in E. coli are similar to those previously described in Y. enterocolitica .
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