Rat Monoclonal Antibodies to Mouse IgG1, IgG2a, IgG2b, and IgG3 Subclasses, and Kappa Chain Isotypic Determinants
1984; Mary Ann Liebert, Inc.; Volume: 3; Issue: 2 Linguagem: Inglês
10.1089/hyb.1984.3.163
ISSN2168-7897
AutoresCHRISTINE LABIT, Michel Pierres,
Tópico(s)Protein purification and stability
ResumoRats of the LOU/Ws1 strain were immunized with mixtures of mouse monoclonal antibodies (MAbs) of various isotypes, and their spleen cells were fused with the rat myeloma Y3.Ag1.2.3 or the mouse myeloma X63.Ag8.653. From four fusion experiments, we have selected 14 rat MAbs that exhibited selective binding to either IgG2a, IgG2b, IgG1, and IgG3 subclasses or to κ chain isotypic determinants. Cross-blocking studies revealed that three rat MAbs identified distinct determinants on the Fc fragment of the MAb H10-81.10 (A.TH, Igh-1e). By contrast, the IgG1, IgG2b, IgG3, and κ isotypes defined by the mAbs analyzed in this study were found to be in close spatial relationship. These rat MAbs bound IgG2a, IgG2b, or IgG1 mouse MAbs, expressing the Igh-1e,j,a, or c, Igh-3a or b, or Igh-4 a or b allelic specificities, respectively.
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