Manipulation of B. megaterium growth for efficient 2-KLG production by K. vulgare
2009; Elsevier BV; Volume: 45; Issue: 4 Linguagem: Inglês
10.1016/j.procbio.2009.11.016
ISSN1873-3298
AutoresJing Zhang, Jie Liu, Zhongping Shi, Li Liu, Jian Chen,
Tópico(s)Pharmacological Effects of Natural Compounds
ResumoIn the two-step Vitamin C fermentative production, its precursor 2-keto-l-gulonic acid (2-KLG) was synthesized by Ketogulonicigenium vulgare through co-culture with Bacillus megaterium. The rates of K. vulgare cell growth and 2-KLG production were closely related with B. megaterium concentration in the co-culture system. To enhance the 2-KLG production efficiency, a strategy of manipulating B. megaterium growth in the co-culture system and properly releasing its intracellular components was introduced. Lysozyme was used specifically to damage B. megaterium cell wall structure and subsequently inhibit its cell growth. When 10,000 U mL−1 lysozyme was fed to the co-culture system at 12 h, the growth rate of K. vulgare, sorbose consumption rate, and 2-KLG productivity could increase 27.4%, 37.1%, and 28.2%, respectively.
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