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Produção de fator de necrose tumoral-alfa e peróxido de hidrogênio na infecção pelo Trypanosoma cruzi em camundongos submetidos ao exercício

2009; UNIVERSIDADE ESTADUAL DE MARINGÁ; Volume: 32; Issue: 1 Linguagem: Inglês

10.4025/actascihealthsci.v32i1.6757

1807-8648

Silvana Márques de Araújo, Cristiano Schebeleski Soares, Roberta Cristhiany Occhi, Luana Gomes Luz de Carvalho, Solange Marta Franzói de Moraes, Márcia Machado de Oliveira Dalálio,

Parasitic Infections and Diagnostics

This study aimed to determine the levels of seric tumor necrosis factor-alpha (TNF-alpha) and production of hydrogen peroxide (H2O2) by peritoneal macrophages in mice experimentally infected with T. cruzi and submitted to pre-infection exercise training and to post-infection acute exercise. Female 30-day-old BALB/c mice were inoculated with 1,400 blood trypomastigotes of Y-strain T. cruzi. Exercise programs consisted in moderate-intensity activity and were carried out in a treadmill. The measurements were performed with material collected at the 13th day after infection Serum TNF-alpha was evaluated using capture ELISA. H2O2 production was expressed by coloration produced after incubation of peritoneal macrophages and the measurement was performed using an ELISA reader. There were no statistically significant differences in TNF-alpha levels and H2O2 production between the trained and non-trained infected groups. Thus, the physical training performed before infection and physical exercise performed after the infection were not able to change the levels of TNF-alpha and production of H2O2 in the infection by T. cruzi.