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Trizol-based method for sample preparation and isoelectric focusing of halophilic proteins

2006; Elsevier BV; Volume: 351; Issue: 2 Linguagem: Inglês

10.1016/j.ab.2006.01.017

1096-0309

P. Aaron Kirkland, Jennifer Busby, Stanley M. Stevens, Julie A. Maupin‐Furlow,

Physiological and biochemical adaptations

A persisting complication in the development of well-resolved two-dimensional PAGE maps of halophilic proteins is their natural incompatibility with isoelectric focusing (IEF). The complete desalting of samples, which is necessary for IEF, tends to aggregate halophilic proteins, often requires relatively large amounts of starting material due to significant loss of sample, and is relatively time-consuming. Here, we describe a method of preparing protein samples from the haloarchaeon Haloferax volcanii that not only desalts the samples thoroughly but also drastically reduces the amount of protein loss associated with previous sample preparation methods and prevents protein aggregation during the removal of salt. This method of sample preparation, which incorporates Trizol (phenol/guanidine isothiocyanate), can easily be extended to analyze halophilic proteins from other organisms.