Extraction of electron acceptor A 1 from pea photosystem I
1987; Wiley; Volume: 220; Issue: 1 Linguagem: Inglês
10.1016/0014-5793(87)80878-5
ISSN1873-3468
AutoresRoyston W. Mansfield, Julia A. Hubbard, Jonathan H. A. Nugent, M.C.W. Evans,
Tópico(s)Photoreceptor and optogenetics research
ResumoPhotosystem I particles were washed twice in either 0, 50 or 100% water‐saturated diethyl ether. It was found that the characteristic electron spin resonance signal associated with electron acceptor A 1 was progressively lost with increasing percentage saturation of the ether. Light‐induced electron flow to the terminal iron‐sulphur acceptors was inhibited although these acceptors were still present and could be chemically reduced. The kinetics of optical measurements of P700 + re‐reduction at 820 nm following ether washing were consistent with removal of electron acceptor A 1 .
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